Application

Anti-CD3e, clone 1H4 ZooMAb, Cat. No. ZRB1011, is a recombinant Rabbit Monoclonal Antibody that specifically targets of CD3e T-cell surface glycoprotein and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Flow Cytometry Analysis: 0.01 µg from a representative lot detected CD3e in Jurkat cell line.Immunohistochemistry (Paraffin) Analysis: A 1:40 dilution from a representative lot detected CD3e in human tonsil tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD3e in Jurkat cell line.Immunofluorescence Analysis: A 1:40 dilution from a representative lot detected CD3e in human tonsil tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human T-cell surface glycoprotein CD3 epsilon chain (CD3e).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

120 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1H4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects CD3e. It targets an epitope within 13 amino acids from the C-terminal, cytoplasmic domain.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

T-cell surface glycoprotein CD3 epsilon chain (UniProt P07766; also known as CD3-epsilon, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain) is encoded by the CD3E (also known as T3E) gene (Gene ID 916) in human. The T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha and beta dimer, while among 5% of T cells the TCR is composed of a gamma and a delta dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The alpha and beta chains have very short cytoplasmic tail and rely on the CD3 (cluster of differentiation 3) co-receptor complex for transducing signaling further intracellularly. The CD3 co-receptor complex is composed of a CD3 chain, a CD3 chain, and two CD3 chains. The CD3 complex, TCR dimer, and a -chain together comprise the functional TCR complex. The CD3 epsilon chain is initially produced with a signal peptide (aa 1-22), the removal of which yields the mature chain (aa. 23-207) with an Ig-like (aa 32-112) domain-containing extracellular region (aa 23-126), a transmembrane segment (aa 127-152), and a cytoplasmic tail (aa 153-207), which contains the immunoreceptor tyrosine-based activation motif or ITAM (a.a. 178-205) that is essential for mediating ZAP-70 interaction and transducing TCR signaling. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.